Plasma Functionalized Scaffolds of Polyhydroxybutyrate Electrospun Fibers for Pancreatic Beta Cell Cultures

Fibrous scaffolds based on biodegradable and biocompatible polyhydroxybutyrate (PHB) were produced by electrospinning. The coated a thin solid film of polypyrrole, PPyI, deposited plasma polymerization technique. FTIR Raman spectroscopy results confirm the presence PPyI PHB fibers, in can be show signal NH2 groups characteristic so broad band due to fluorescence from coating. morphology was characterized scanning electron microscopy, average fiber diameter is (2.68 ± 1.69) ?m, also fibers PHB-PPyI (3.57 1.36) comparation uncoated indicates that thickness 0.45 ?m. Crystallinity X-ray diffraction differential calorimetry, degree crystallinity estimated at 70%. Pancreatic beta cells (from RIN-M cell line) cultured scaffolds. Cell viability showed surface modified material good culture substrate for cells.